Bart, you've got the right equipment for this piece of reverse engineering. I don't have a LensAlign ruler. What are the dimensions in mm of the gradations that you show?
I assume you focused on the 0 point (the bright white line) in each shot? And what is the depth of focus on the lensalign scale for a single shot? (In my 80 mm f/2.8 test, it was about 5 cm.) I wonder if the focus changes that they make are keyed to the depth of field (which would mean a dependance on focal length and on aperture) or are just fixed distances. To test this would require shooting with the 60 macro at, say 5.6, stacking with 1 unit, and then with the zoom at maybe 18mm, f/2.8. Want to try it?
scott
EDIT: Notice when you set the increment to 5, the steps were bigger than the depth of field of a single shot, leaving blurry portions at +20, +7.5 -5 and -14. I bet if you ran 5 units at f/5.6, those blurs would sharpen right up, and the total area covered would stay the same -- meaning that the units are actually lengths. That still leaves the question of whether the lengths are scaled to the distance at which you initially focus. Oh well, experiments still needed to use this.